Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZMAT2 in samples. An antibody specific for ZMAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZMAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: ZMAT2, Contains 1 matrin-type zinc finger. ZMAT2 encodes a zinc finger protein. Alternatively spliced transcript variants have been described. Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression.
Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules. The name "zinc finger" was originally coined to describe the hypothesized structure of the repeated unit in Xenopus laevis transcription factor IIIA